Neutralizers that inactivate the disinfectants shall be included either in the diluent or microbiological media used for microbial enumeration.Neutralizing agents for Disinfectants and Sanitizing agents (Efficacy Testing):.Safety consideration to the operators applying the disinfectant or sanitizing agents.The corrosiveness of the disinfectant to equipment with repeated application.The possible need to maintain a residual bactericidal activity of the disinfectant on the surface.The amount of organic compounds on the surface that may inactivate a disinfectant or sanitizing agent.Nature of the surface material and its compatibility with the disinfectant or sanitizing agents.The concentration, application method and contact time with the disinfectant or sanitizing agent.The disinfectant or sanitizer supporters by the EPA registrations.The spectrum of activity of commercially available disinfectants and sanitizing agents.Number and types of microorganisms to be controlled.The Following points to be consider for selection of disinfectants and sanitizing agents for efficacy testing.Selection Criteria of Disinfectants and Sanitizing Agents foe Efficacy Testing:.General purpose disinfectant (bactericide), antiseptic Vapour phase sterilant, liquid sporicidal agent, antisepticĤ µg per g H2O2 vapour, 10%-25% solution, 3% solution.Ġ.2% Peracetic acid, 1 µg per g Peracetic acid General purpose disinfectant (Bactericide), antiseptic, antiviral agentĥ00 µg per g chlorocresol, 500 µg per g chloroxylenol. These include aldehydes, alcohols, halogens, peroxides, quaternary compound (QAC) and phenolic compounds (see Table-02).Chemically disinfectants are classified by their chemical type.Classification of Disinfectant and Sanitizing Agents:.However, all the environmental isolates, which were isolated from an environmental monitoring program shall be challenged to the efficacy evaluation of disinfectants/sanitizing agents to confirm their susceptibility, other wise most frequently isolated microorganisms is also acceptable.Spore forming bacteria and Gram +ve bacilli. Vegetative Bacteria and Gram +ve cocci in clusters. Vegetative Bacteria and Gram –ve small bacilli Name of the challenge Microorganism (Standard Test Organism) The typical challenge microorganisms that can be employed are listed in Table-01.For the studies on disinfectants used for spraying and surface disinfection isolates selected must involve spore bearing prokaryotic and eukaryotic microorganisms.Selection of ATCC and Environmental isolates is done to cover the entire microorganism based on the gram character and cell morphology.Challenge Microorganisms – Disinfectants Efficacy Measurement:.Tryptone Soya Agar with Lecithin and Tween-80 or Dey/Engley (D/E) agar.Pre-Requisites for Disinfectant Efficacy Testing.USP Chapter Disinfectants and Antiseptics.Ħ.0 METHODOLOGY – Four Level of Disinfectants Efficacy:.
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